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imaging buffer  (Thermo Fisher)


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    Thermo Fisher imaging buffer
    Imaging Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imaging+buffer/HEPES/pm41984033-78-24-85
    Average 99 stars, based on 1 article reviews
    imaging buffer - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Imaging:

    Article Title: WNK-Dependent Phosphorylation of Gephyrin Tunes GABA A Receptors at Inhibitory Synapses and Modulates Anxiety Behavior
    Article Snippet: 1.49) and an Andor iXon Ultra 897 EMCCD camera (image pixel size, 160 nm) (Oxford Instruments, Abingdon, UK), using specific lasers for STORM imaging of Alexa 647 (640 nm). .. Samples were imaged in a PBS-based oxygen-scavenging buffer containing imaging buffer (Tris 100 mM Invitrogen, NaCl 20 mM, pH 8), glucose 40% (w/v, Sigma-Aldrich), PBS 1X, cysteamine hydrochloride (77 mg/mL, Sigma-Aldrich), catalase 5 mg/mL (Sigma-Aldrich) and glucose oxidase 67.6 U/mL (Sigma-Aldrich). .. Catalase was diluted in MgCl 4 mM, EGTA 2 mM, and PIPES 24 mM (Sigma-Aldrich, pH 6.8).

    Article Title: Early Aβ-induced changes in the enteric nervous system and the gut: structure, function, and motility
    Article Snippet: Following the washing steps, the coverslip with the cells was fixed and aligned in the perfusion chamber RC-20 (Warner Instruments) for live-cell imaging. .. Measurements were performed under continuous perfusion with physiological imaging buffer (148mM NaCl, 5mM KCl, 2mM CaCl 2 , 1mM MgCl 2 , 10mM glucose, and 10mM HEPES [pH 7.38, all Thermo Fisher Scientific]), either alone or supplemented with Aβ (10nM, 100nM, or 1μM). .. Calcium imaging was conducted using a Zeiss CellObserver Z1 and the Axiovision software (Rel.

    Article Title: Maximizing photon utilization in spectroscopic single-molecule localization microscopy using symmetrically dispersed dual-wedge prisms.
    Article Snippet: .. For STORM imaging, we used an imaging buffer consisting of 50mM Tris-HCl (J22638.AP; ThermoFisher), 10mM NaCl (BDH8014; BDH), 0.5mg/mL glucose oxidase (G2133; Sigma Aldrich), 2000 U/mL catalase (C30; Sigma Aldrich), 10% (w/v) D-glucose (A16828.36; ThermoFisher), and 100mM cysteamine (M9768-5G; Sigma Aldrich) at approximately pH 8.0. ..

    Article Title: Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau.
    Article Snippet: For capturing DNA samples, 8 nM NeutrAvidin (Thermo #31000) was introduced into the flow cell, incubated for 5 min, and then washed with PBS. .. The flow cell surface was further passivated by infusing 5% BSA (Sigma #A0100-010) for 10 min, followed by another PBS wash. Biotinylated λ-DNA was diluted to 10–20 pM, injected, and immobilized on the surface for 7 min. For imaging, the buffer was exchanged with 200 μL of imaging buffer (50 mM HEPES (pH 7.1), 100 mM NaCl, 2 mM Trolox, 59.5 nM protocatechuate 3,4-dioxygenase (PCD #P8279-25UN), and 2.5 mM protocatechuic acid (PCA #37580-25G-F)) containing 20 nM SYTOX Orange (Thermo #S11368) and incubated for 10 min. To examine tau–DNA co-condensation, tau protein was diluted in the imaging buffer to concentrations ranging from 50 nM to 5 μM and then injected into the flow cell. ..

    Article Title: Defects in skeletal myotubes caused by STIM1 I115F that lead to tubular aggregate myopathy and Stormorken syndrome and their restoration at the cellular level.
    Article Snippet: .. 211 Live single-cell Ca2+ imaging experiments 212 As previously described (25, 35, 36), myotubes were loaded with 5 μM Fluo-4 AM (Invitrogen) in 213 imaging buffer (25 mM HEPES, 125 mM NaCl, 5 mM KCl, 2 mM KH2PO4, 2 mM CaCl2, 6 mM glucose, 214 1.2 mM MgSO4, and 0.05% BSA; pH 7.4) for 45 min at 37 °C for all the live single-cell Ca2+ imaging 215 experiments except for the measurement of cytosolic Ca2+ levels, which was performed with 5 μM Fura-2 216 AM (Invitrogen). .. Live single-cell Ca2+ imaging was performed with a high-speed monochromator 217 (FSM150Xe with a 75 W xenon lamp; Bentham Instruments, Reading, Berkshire, UK) and an inverted 218 stage microscope (Nikon Eclipse TS100; Nikon Instruments).

    Article Title: Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau
    Article Snippet: For capturing DNA samples, 8 nM NeutrAvidin (Thermo #31000) was introduced into the flow cell, incubated for 5 min, and then washed with PBS. .. The flow cell surface was further passivated by infusing 5% BSA (Sigma #A0100-010) for 10 min, followed by another PBS wash. Biotinylated λ-DNA was diluted to 10–20 pM, injected, and immobilized on the surface for 7 min. For imaging, the buffer was exchanged with 200 μL of imaging buffer (50 mM HEPES (pH 7.1), 100 mM NaCl, 2 mM Trolox, 59.5 nM protocatechuate 3,4-dioxygenase (PCD #P8279-25UN), and 2.5 mM protocatechuic acid (PCA #37580-25G-F)) containing 20 nM SYTOX Orange (Thermo #S11368) and incubated for 10 min. To examine tau–DNA co-condensation, tau protein was diluted in the imaging buffer to concentrations ranging from 50 nM to 5 μM and then injected into the flow cell. ..

    Article Title: Structural Basis of Condensin Recruitment for X Chromosome Repression
    Article Snippet: .. After assembly, flow chambers were flushed with 25 μg/mL streptavidin (Merck) in rinsing buffer (50 mM TRIS-HCl pH 7.5, 20 mM KOAc) and rinsed thoroughly before introducing 1–10 pM biotinylated λ-phage DNA in imaging buffer (50 mM TRIS-HCl pH 7.5, 50 mM KOAc, 2.5 mM MgCl 2 , 5 % (w/v) D-glucose, 1 mM DTT, 500 nM Sytox orange (SxO, ThermoFisher), 40 μg/mL glucose oxidase (Sigma), 15 μg/mL catalase (Sigma), 2 mM Trolox (Sigma), 0.5 mg/mL BSA (NEB)) with a continuous flow of 6–8 μL/min using a PHD2000 syringe pump (Harvard Apparatus). ..

    other:

    Article Title: The force-sensing GPCR LPHN2 is indispensable for normal auditory function.
    Article Snippet: Data are recorded and analyzed using the VisiFLUOR Fluorescence Ratio Imaging System (Visitron Systems).

    Injection:

    Article Title: Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau.
    Article Snippet: For capturing DNA samples, 8 nM NeutrAvidin (Thermo #31000) was introduced into the flow cell, incubated for 5 min, and then washed with PBS. .. The flow cell surface was further passivated by infusing 5% BSA (Sigma #A0100-010) for 10 min, followed by another PBS wash. Biotinylated λ-DNA was diluted to 10–20 pM, injected, and immobilized on the surface for 7 min. For imaging, the buffer was exchanged with 200 μL of imaging buffer (50 mM HEPES (pH 7.1), 100 mM NaCl, 2 mM Trolox, 59.5 nM protocatechuate 3,4-dioxygenase (PCD #P8279-25UN), and 2.5 mM protocatechuic acid (PCA #37580-25G-F)) containing 20 nM SYTOX Orange (Thermo #S11368) and incubated for 10 min. To examine tau–DNA co-condensation, tau protein was diluted in the imaging buffer to concentrations ranging from 50 nM to 5 μM and then injected into the flow cell. ..

    Article Title: Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau
    Article Snippet: For capturing DNA samples, 8 nM NeutrAvidin (Thermo #31000) was introduced into the flow cell, incubated for 5 min, and then washed with PBS. .. The flow cell surface was further passivated by infusing 5% BSA (Sigma #A0100-010) for 10 min, followed by another PBS wash. Biotinylated λ-DNA was diluted to 10–20 pM, injected, and immobilized on the surface for 7 min. For imaging, the buffer was exchanged with 200 μL of imaging buffer (50 mM HEPES (pH 7.1), 100 mM NaCl, 2 mM Trolox, 59.5 nM protocatechuate 3,4-dioxygenase (PCD #P8279-25UN), and 2.5 mM protocatechuic acid (PCA #37580-25G-F)) containing 20 nM SYTOX Orange (Thermo #S11368) and incubated for 10 min. To examine tau–DNA co-condensation, tau protein was diluted in the imaging buffer to concentrations ranging from 50 nM to 5 μM and then injected into the flow cell. ..

    Incubation:

    Article Title: Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau.
    Article Snippet: For capturing DNA samples, 8 nM NeutrAvidin (Thermo #31000) was introduced into the flow cell, incubated for 5 min, and then washed with PBS. .. The flow cell surface was further passivated by infusing 5% BSA (Sigma #A0100-010) for 10 min, followed by another PBS wash. Biotinylated λ-DNA was diluted to 10–20 pM, injected, and immobilized on the surface for 7 min. For imaging, the buffer was exchanged with 200 μL of imaging buffer (50 mM HEPES (pH 7.1), 100 mM NaCl, 2 mM Trolox, 59.5 nM protocatechuate 3,4-dioxygenase (PCD #P8279-25UN), and 2.5 mM protocatechuic acid (PCA #37580-25G-F)) containing 20 nM SYTOX Orange (Thermo #S11368) and incubated for 10 min. To examine tau–DNA co-condensation, tau protein was diluted in the imaging buffer to concentrations ranging from 50 nM to 5 μM and then injected into the flow cell. ..

    Article Title: Tau condensation on DNA mediates microtubule attachment suggesting a mitotic role for centromere-localized tau
    Article Snippet: For capturing DNA samples, 8 nM NeutrAvidin (Thermo #31000) was introduced into the flow cell, incubated for 5 min, and then washed with PBS. .. The flow cell surface was further passivated by infusing 5% BSA (Sigma #A0100-010) for 10 min, followed by another PBS wash. Biotinylated λ-DNA was diluted to 10–20 pM, injected, and immobilized on the surface for 7 min. For imaging, the buffer was exchanged with 200 μL of imaging buffer (50 mM HEPES (pH 7.1), 100 mM NaCl, 2 mM Trolox, 59.5 nM protocatechuate 3,4-dioxygenase (PCD #P8279-25UN), and 2.5 mM protocatechuic acid (PCA #37580-25G-F)) containing 20 nM SYTOX Orange (Thermo #S11368) and incubated for 10 min. To examine tau–DNA co-condensation, tau protein was diluted in the imaging buffer to concentrations ranging from 50 nM to 5 μM and then injected into the flow cell. ..

    Single Cell:

    Article Title: Defects in skeletal myotubes caused by STIM1 I115F that lead to tubular aggregate myopathy and Stormorken syndrome and their restoration at the cellular level.
    Article Snippet: .. 211 Live single-cell Ca2+ imaging experiments 212 As previously described (25, 35, 36), myotubes were loaded with 5 μM Fluo-4 AM (Invitrogen) in 213 imaging buffer (25 mM HEPES, 125 mM NaCl, 5 mM KCl, 2 mM KH2PO4, 2 mM CaCl2, 6 mM glucose, 214 1.2 mM MgSO4, and 0.05% BSA; pH 7.4) for 45 min at 37 °C for all the live single-cell Ca2+ imaging 215 experiments except for the measurement of cytosolic Ca2+ levels, which was performed with 5 μM Fura-2 216 AM (Invitrogen). .. Live single-cell Ca2+ imaging was performed with a high-speed monochromator 217 (FSM150Xe with a 75 W xenon lamp; Bentham Instruments, Reading, Berkshire, UK) and an inverted 218 stage microscope (Nikon Eclipse TS100; Nikon Instruments).



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    Image Search Results


    a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: bioRxiv

    Article Title: Mouse behavioral genomics identifies Creld1 as a gatekeeper of somatosensation

    doi: 10.64898/2026.03.30.715210

    Figure Lengend Snippet: a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: In brief, cells were washed with the Ca 2+ imaging buffer (1 x HBSS buffer with 1.3 mM Ca 2+ , 10 mM HEPES, pH 7.2) and then loaded with the Ca 2+ imaging buffer containing 2.5 mM Ca 2+ indicator dye Fura-2, AM (ThermoFisher scientific, F1225) and 0.05% Pluronic F-127 (Beyotime, ST501).

    Techniques: CRISPR, Knock-Out, Injection, Virus, Construct, Infection, Single Cell, Imaging, Derivative Assay, Control, Cell Culture, Randall–Selitto Test, Hot Plate Test